Ask A Scientist: What’s the Difference Between M1 and M2 Macrophages?

ask a scientistMacrophages are a diverse group of white blood cells known for eliminating pathogens through phagocytosis. In the past, macrophages were classified by the organ in which they were found: Kuppfer cells in the liver, Langerhans cells in the skin, microglia in the brain and spinal cord, osteoclasts in the bone.

Scientists continue to debate whether macrophages originate in each organ or migrate there from the bone marrow. To clear up some of this debate, the current taxonomy has shifted away from organ-specific macrophages to M1 and M2 macrophages.

Defining M1 and M2 Macrophages

This classification is based upon macrophage polarization rather than macrophage location.

M1 macrophages are classically activated, typically by IFN-γ or lipopolysaccharide (LPS), and produce proinflammatory cytokines, phagocytize microbes, and initiate an immune response. M1 macrophages produce nitric oxide (NO) or reactive oxygen intermediates (ROI) to protect against bacteria and viruses.

M2 macrophages are alternatively activated by exposure to certain cytokines such as IL-4, IL-10, or IL-13. M2 macrophages will produce either polyamines to induce proliferation or proline to induce collagen production. These macrophages are associated with wound healing and tissue repair.

There are three types of M2 macrophages: M2a, M2b, and M2c. Click the image to learn more.

Defining M1 and M2 Macrophages- Astarte Bio
Source: Tamás Rőszer, “Understanding the Mysterious M2 Macrophage through Activation Markers and Effector Mechanisms,” Mediators of Inflammation, vol. 2015, Article ID 816460, 16 pages, 2015. doi:10.1155/2015/816460

M2 macrophages also contribute to the formation of extracellular matrix and do not produce nitric oxide or present antigen to T cells. Tumor-infiltrating macrophages are typically classified as M2, although some classify them as myeloid-derived suppressor cells (MDSC).

What’s the Difference Between M1 and M2 Macrophages? -Astarte Biologics

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6 thoughts on “Ask A Scientist: What’s the Difference Between M1 and M2 Macrophages?

  1. Kenneth Allan Brasel says:

    I would make one comment on your M1/M2 article. While M1 are known for tumoricial activity mostly due to NO- production (some of it is due to TNFa), thats only for mouse/rodent macrophage. Human macrophage stimulated the same way (typically IFNg and LPS) do not secrete appreciable NO- nor kill near as well as mouse macrophage. Many of those reports came out around 1993.

  2. Anne Lodge says:

    Hi Ken, thanks for adding that in. I know you are a macrophage enthusiast so I appreciate you pointing out the differences between human and mouse macrophages.

  3. Marek says:

    Would it be possible to perform immunofluorescence using arginase-1 and iNOS in mouse tissue samples in order to distinguish M1 and M2 subpopulations? or is it better to use cd80 and cd206?

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