Ask A Scientist:
Should I Get CD34+ Stem Cells from Bone Marrow, Cord Blood, or Peripheral Blood?

ask a scientist

ask a scientist

You might know that CD34+ stem cells can be sourced from three different locations: bone marrow, cord blood, and peripheral blood. But does it matter which source you use for your CD34+ cells? And do you know the different functions and best use cases for each?

We’ll help you find the right stem cell source for your research.

CD34+ Stem Cells from Bone Marrow

Bone marrow is the natural habitat for hematopoietic stem cells; however, the collection process is quite invasive. Researchers often obtain their CD34+ cells from more easily accessible blood sources. Our bone marrow-derived CD34+ cells are purified to remove the lipids and bony spicules typically found in bone marrow aspirates.

CD34+ Stem Cells from Cord Blood

Stem cells sourced from umbilical cord blood have a much higher cell count, greater capacity for cell division, and longer lifespan than CD34+ cells from adult bone marrow. With cord blood stem cells, you should be able to get more rounds of cell division (self-renewal) from your sample, so this may be the right source if you plan to use the cells in long-term cultures.

CD34+ Stem Cells from Peripheral Blood

Peripheral blood is by far the most accessible source of CD34+ cells, and the collection process is much less invasive. However, the concentration of CD34+ cells in circulating peripheral blood is much lower than in bone marrow or cord blood, so this is not the best source if your research requires a large number of CD34+ cells.

Stem cells are not naturally found in peripheral blood in circulation but can get there through cytokine mobilization. Mobilized peripheral blood has become the most common source of hematopoietic stem cells intended for transplantation.

The percentage of CD34+ cells and the overall number of cells that can be collected are elevated in peripheral blood, allowing for the highest number of CD34 possible in a single lot.

 

Unlimited Possibilities with CD34+ Cells

In addition to the benefits outlined above, CD34+ cells have the multipotency to create other cell types that can be difficult to source. In theory, you should be able to turn CD34+ cells into nearly any other blood cell type, although some formulations are not yet known.

Well-known conversion types include CD34+ cells to mast cells, dendritic cells, and other myeloid cell types.

Have more questions about CD34+ cells? Ask an Astarte scientist for help!

2 thoughts on “Ask A Scientist:
Should I Get CD34+ Stem Cells from Bone Marrow, Cord Blood, or Peripheral Blood?

    • Avatar
      Anne Lodge says:

      Hi Chanchai, thanks for joining the discussion. There are serum free and reduced serum media but you are right to point out that they are more expensive than the older formulations. I would recommend Iscove’s modified Dulbecco medium (IMDM) as a better choice than RPMI 1640. It has more nutrients that support growth of hematopoietic stem cells and is less expensive than the newer formulations.

Leave a Reply

Your email address will not be published. Required fields are marked *

This site uses Akismet to reduce spam. Learn how your comment data is processed.