About AntigensVIEW PRODUCT LISTING
We offer various antigens to stimulate cells, all tested for bioactivity using our PBMC. Our three recall antigens can be used to study different types of immunological memory: Cytomegalovirus (CMV), tetanus, and M1.
PBMC activation by CMV antigen usually indicates a donor who is seropositive for CMV, though we have observed stimulation of PBMC from seronegative donors. CMV establishes a latent infection and is contained by the immune system in healthy CMV positive donors. This results in a relatively large population of CMV reactive T lymphocytes, both CD4+ and CD8+.
The tetanus antigen is complexed with alum but stimulates PBMC proliferation well. Most individuals are vaccinated with this antigen and therefore have an immune response to it. The magnitude of the response varies among donors depending on their immune status, age and time since their last tetanus vaccination. Because of the presence of alum, this preparation may not be suitable for uses other than in vitro immune responses.
The M1 peptide is an immunodominant epitope from influenza virus, but only for donors who express HLA-A*0201. We have observed reactivity to this antigen preparation, but only after a 7–10 day culture period. Unlike CMV, influenza virus is cleared from the body following infection, so the immune response to the antigen wanes after infection. Also, this antigen will only stimulate CD8+ T cells, not CD4+ T cells.
As we add antigen-specific T cells we are also adding their cognate peptide antigens. This includes a peptide from CMV that binds to HLA-A*0201 and a peptide from myelin basic protein that is the epitope recognized by our BC3 T cell line.