About Neutrophils

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Neutrophils are the first responders of the body’s defense system where they rapidly localize to areas of acute infection and phagocytize bacteria. Their cytoplasmic granules contain proteases and are capable of generating reactive oxygen intermediates to kill invading organisms. Neutrophils are the most abundant white blood cell in circulation but have a half-life of only 1–2 days. Pillay, et al. reported data showing a half-life of 5.4 days, but this has been disputed.

Our neutrophils are isolated from freshly obtained blood via negative selection and cryopreserved within a few hours of collection. Phagocytosis has been observed with these cells but we have not been able to detect an oxidative burst following stimulation with phorbol myristate acetate.

Thawing Neutrophils

phagocytosis of fluorescently labeled bacteria by neutrophils following our Neutrophil Phagocytosis Assay.Be sure to review the instructions for thawing neutrophils, as these cells require special handling.

The figure shows phagocytosis of fluorescently labeled bacteria by neutrophils following our Neutrophil Phagocytosis Assay. The gray histogram represents cells with no bacteria, the blue histogram shows cells with non-opsonized bacteria, and the red histogram represents cells that have ingested opsonized bacteria.

Many thanks to Kristy Katzenmeyer, Ph.D., of the University of Washington for allowing us to display her data.