About Normal PBMCVIEW PRODUCT LISTING
Our peripheral blood mononuclear cells (PBMC) are collected by leukapheresis and further processed using Ficoll gradient and several washes to remove platelets. We test all our lots post-thaw for in vitro responses to CMV antigens, tetanus toxoid and LPS.
Our normal PBMC products are HLA typed so you can select your HLA type of interest. Our PBMC express a variety of alleles from the always-popular HLA-A*0201 to B*2705, DRB1*0401, and other alleles common in the U.S. population. All donors are tested negative for HIV, Hepatitis B and C, and other blood-borne pathogens. Our healthy donors may take low-dose aspirin, birth control pills or anti-depressants, but many do not take medications.
We are genotyping donors for the SNP at position 158 in Fc gamma RIII alpha, which is provided in the Certificate of Analysis when available. All cells should be handled as if potentially infectious following biosafety level 2 procedures.
Store normal PBMC in the vapor phase of liquid nitrogen (< –120°C). Product may be stored at < –75°C for several days, but cell viability will decrease with extended storage.
Publications Using PBMC from Astarte Biologics
Some mechanisms of FLIP expression in inhibition of HIV-1 replication in Jurkat cells, CD4+ T cells and PBMCs. Journal of Cellular Physiology. 2013.
The design, selection, and evaluation of highly specific and functional siRNA incorporating unlocked nucleobase analogs. Methods in Molecular Biology. 2013.
LXR-mediated inhibition of CD4+ T helper cells. PLOS ONE. 2012.
Engineering a CD123xCD3 bispecific scFv immunofusion for the treatment of leukemia and elimination of leukemia stem cells. Protein Engineering Design and Selection. 2012.
Digital PCR provides absolute quantification of viral load for an occult RNA virus. Journal of Virological Methods. 2011.
HIV-1 co-infection and morphine co-exposure severely dysregulate HCV-induced hepatic pro-inflammatory cytokine release and free radical production: increased pathogenesis coincides with uncoordinated host-defenses. Journal of Virology. 2011.
PLTP Regulates STAT3 and NFκB in differentiated THP-1 cells and human monocyte-derived macrophages. Biochimica et Biophysica Acta. 2011.
Novel cancer vaccine based on genes of Salmonella pathogenicity island 2. International Journal of Cancer. 2010.