About CD4+ T Cells

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Our CD4+ T cells are purified from PBMC using negative immunomagnetic selection.

CD4+ T cells make up 2/3 of the T cells in peripheral blood. While they were originally considered helper T cells, we now know that there are subsets within the CD4+ population with distinct functions.

Antigen-Specific CD4+ T Cells are polyclonal T cell lines derived by repeated activation with antigen presenting cells and peptide antigen. We verify the response of the T cells to the peptide antigen of interest and determine the HLA-DRB1 allele responsible for presenting the antigen to the T cells. These T cells can be used in assays on the day they are thawed for ease of use in your experiments.

Naïve CD4+ T Cells are those cells that have not encountered their cognate antigen and do not have a defined function. They are shown to express CD45RA, as expected in this T cell population.

Memory CD4+ T Cells are those cells that have engaged with a peptide antigen bound to an MHC class II molecule. They have a particular function such as follicular helper cells, Th1 providing cytokines for cytotoxic T cells, or Th2 supporting differentiation of B cells.

Total CD4+ T Cells contain both naïve and memory T cells and are ideal for mixed lymphocyte cultures or any assay measuring the response to an exogenous antigen.

We have CD4+ T cells from a variety of donors. All donors are HLA typed and tested negative for blood-borne pathogens.

Publications Using T Cells from Astarte Biologics

Quantifying the cluster of differentiation 4 receptor density on human T lymphocytes using multiple reaction monitoring mass spectrometry. Analytical Chemistry. 2013.

Hepcidin induces HIV-1 transcription inhibited by ferroportin. Retrovirology. 2010.

Generation of nondividing high rate Ig-secreting plasma cells in cultures of human B cells stimulated with anti-CD3-activated T cells. Journal of Immunology. 1992.