Immune Cell Protocols

Helping You Get on with Discovery

The protocols listed here have been used in our lab using our cells, so we know they work. You may need to adjust conditions in your lab.

If you have any questions along the way, you can always call us to discuss these and other techniques. We’re always checking new media formulations and trying to improve cell performance, so check back periodically to see the latest.

Antigen-Specific T Cell Assay

Our antigen-specific T cells can be used to measure the effects of drugs and antibodies on the immune response. Here’s the basic method for measuring T cell activation in vitro.

How to Thaw Neutrophils

Neutrophils require a different approach to cell thawing and handling because they are so easily activated and damaged.

CFSE Labeling

Labeling cells with carboxyfluorescein succinimidyl ester (CFSE) is an elegant technique for measuring cell proliferation.

Human Dendritic Cell Culture

Dendritic cells do not divide but they can be cultured and activated. You just need to keep these tips in mind.

Chondrocyte Culture

Chondrocyte proliferation is required to build cartilage for joint repair. This is a basic description of an easy measurement of chondrocyte proliferation.

Measurement of Cartilage Synthesis

Chondrocytes will synthesize cartilage in vitro. This protocol describes how to measure it and how to modulate it.

Neutrophil Phagocytosis Assay

This method has been used to measure phagocytosis by neutrophils.

Culture of B-Lymphoblastoid Cell Lines

Our B-lymphoblastoid cell lines (B-LCL) are easy to grow, but instructions are still helpful.

Phagocytosis

This is a different phagocytosis protocol using monocytes. We’ve used this protocol to observe phagocytosis by fluorescence microscopy.

Culture of Human Monocytes

Human monocytes can be cultured to create macrophages or dendritic cells. Culture conditions affect cell function and phenotype, so we can give you some pointers.

Recall Antigen Testing

This cytokine release assay is the method we use to test the function of our PBMC. It can be adapted to measure responses to other recall antigens, too.

Culture of Mouse Bone Marrow Cells

This protocol describes how to convert mouse bone marrow cells to macrophages, dendritic cells, or mast cells.

Treg Suppression Assay

This is the method we use to demonstrate the suppressive activity of our T regulatory cells, which work right from the freezer.

Mouse Mast Cell Culture

This protocol describes the materials and methods we use to culture mouse mast cells.

How to Thaw Cells

Thawing cells is simple, but speed is key to optimum cell recovery. Use these tips.